Making clear and durable hemaglobin preparations.



No Drawing.

UNITED Tl OFFICE 'rHILiPr LIWY. orimnLm-wrnmnnsnonr; ennmm;

MAKING 0mm in nimaBLE HEMAGLQIBIN i imraxwrrous;

pound of the red blood corpuscles after being liberated from theserum-and clarified,

In the manufacture of hema-globin prepa-, rations the following process,is generally resorted toqThe cloudy hemaglobin is first clarified bymeans of. ether, chloroform,

spirit, or the like, the clarifier being after-' ward separated from thehemaglobin preparat-ion by heating or by any other suitable method; -Theclarified hemaglobin solution is thereupon preserved by adding to thesame a suitable quantity of glycerin. It is evident that the bloodcorpuscles which are easily decomposed, are not favorably affected bythese numerous reactions. The color of the hemaglobin, for instance,which is origi-, 'nally red, is turned-into browmsh-red by. the process,2. e., aconversion to oxyhema-- globin and met-hemaglobin takes place.The present invention relates to a process of clarifying-andpreservingthe hemaglobin which process, apart from being simplerthan the ordinaryprocesses, retains the' original color of the hemaglobin.

The viscid and cloudy hemaglobin, sepa- Speiflcatioirof Letters Iatent.Application'filed March 1a, 1908. Serial No. 421,304.

' Patented Dec. 24,1912.

rated from theserum by centrifugal action,

is directlymixed with a quantity of chemically pure glycerinand thencentrifuged .tovgetherywith the-latter so that an intimate contact ofthe different molecules takes place. The glycerin, of which, owing tothecen- ,trifugal action towhich the solution is sub- 'jected, only asmall quantity is required, clarifies the hemaglobin at the same time asit serves as a preservative for'the same.

For instance 100 parts of hemaglobin-aremixed with 25 parts of glycerin.v The mixture havingv passedthe centrifuge is perfectly clear, but noseparation of glycerin has. taken place. In this manner the hema globinis only subjected to one single reaction and. it retains its origlnalred color.

Therefore the present process is particularly suitablein'the preparationof such extracts which,- owing to the small quantity of glycerin needed,can .be made to contQin a larger percentage of hemaglobin, 4 I-claim:

A process for making clear, hemaglobincontaining preparation, consistingin separatingthe blood corpuscles from the serum bv centrifugal action,then mixing the clarified mass with arelatively small quantity ofglycerin, centrifuging this mixture, substan tiallyas and for thepurpose set forth.

In testimony whereof I haveherelmto set my hand in the presence of twowitnesses.

PHILIPP LEWX.

"Witnesses:

HENR Hasrrm, Wonnnmaa

